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Human Carboxypeptidase Z / CPZ Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human Carboxypeptidase Z / CPZ Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CPZ antibodies →
$153.00each
Catalog Number: LS-G75005-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Carboxypeptidase Z(CPZ) (NM_001014448) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Carboxypeptidase Z | CPZ | Metallocarboxypeptidase Z
Origin Species
Human
NCBI GenBank
NM_003652
SwissProt
Q66K79

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.63 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPPPPPLLLL TVLVVAAARP GCEFERNPAG ECHRPPAADS ATCVDLQLRT CSDAAYNHTT
61FPNLLQHRSW EVVEASSEYI LLSVLHQLLE GQCNPDLRLL GCAVLAPRCE GGWVRRPCRH
121ICEGLREVCQ PAFDAIDMAW PYFLDCHRYF TREDEGCYDP LEKLRGGLEA DEALPSGLPP
181TFIRFSHHSY AQMVRVLRRT ASRCAHVART YSIGRSFDGR ELLVIEFSSR PGQHELMEPE
241VKLIGNIHGN EVAGREMLIY LAQYLCSEYL LGNPRIQRLL NTTRIHLLPS MNPDGYEVAA
301AEGAGYNGWT SGRQNAQNLD LNRNFPDLTS EYYRLAETRG ARSDHIPIPQ HYWWGKVAPE
361TKAIMKWMQT IPFVLSASLH GGDLVVSYPF DFSKHPQEEK MFSPTPDEKM FKLLSRAYAD
421VHPMMMDRSE NRCGGNFLKR GSIINGADWY SFTGGMSDFN YLHTNCFEIT VELGCVKFPP
481EEALYTLWQH NKESLLNFVE TVHRGIKGVV TDKFGKPVKN ARISVKGIRH DITTAPDGDY
541WRLLPPGIHI VIAQAPGYAK VIKKVIIPAR MKRAGRVDFI LQPLGMGPKN FIHGLRRTGP
601HDPLGGASSL GEATEPDPLR ARRQPSADGS KPWWWSYFTS LSTHRPRWLL KY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

652 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.