LSBio
Human CAPN9 / Calpain 9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human CAPN9 / Calpain 9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all CAPN9 antibodies →
$220.00each
Catalog Number: LS-G80318-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Calpain 9(CAPN9) (NM_016452) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Calpain 9 | CAPN9 | GC36 | NCL-4 | NCL4 | Protein CG36 | New calpain 4 | Calpain 9 (nCL-4) | Calpain-9 | Novel calpain large subunit-4
Origin Species
Human
NCBI GenBank
NM_006615
SwissProt
O14815

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPYLYRAPGP QAHPVPKDAR ITHSSGQSFE QMRQECLQRG TLFEDADFPA SNSSLFYSER
61PQIPFVWKRP GEIVKNPEFI LGGATRTDIC QGELGDCWLL AAIASLTLNQ KALARVIPQD
121QSFGPGYAGI FHFQFWQHSE WLDVVIDDRL PTFRDRLVFL HSADHNEFWS ALLEKAYAKL
181NGSYEALKGG SAIEAMEDFT GGVAETFQTK EAPENFYEIL EKALKRGSLL GCFIDTRSAA
241ESEARTPFGL IKGHAYSVTG IDQVSFRGQR IELIRIRNPW GQVEWNGSWS DRMAFKDFKA
301HFDKVEICNL TPDALEEDAI HKWEVTVHQG SWVRGSTAGG CRNFLDTFWT NPQIKLSLTE
361KDEGQEECSF LVALMQKDRR KLKRFGANVL TIGYAIYECP DKDEHLNKDF FRYHASRARS
421KTFINLREVS DRFKLPPGEY ILIPSTFEPH QEADFCLRIF SEKKAITRDM DGNVDIDLPE
481PPKPTPPDQE TEEEQRFRAL FEQVAGEDME VTAEELEYVL NAVLQKKKDI KFKKLSLISC
541KNIISLMDTS GNGKLEFDEF KVFWDKLKQW INLFLRFDAD KSGTMSTYEL RTALKAAGFQ
601LSSHLLQLIV LRYADEELQL DFDDFLNCLV RLENASRVFQ ALSTKNKEFI HLNINEFIHL
661TMNI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

664 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.