Human C1orf228 Protein (Recombinant Myc-DDK (Flag))
Catalog Number: LS-G73705-20
20 µg / $1130.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human C1orf228 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G95953-20
Catalog Number: LS-G95953
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C1orf228 (NM_001145636) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001145636
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSIKEQAAI SRLLSFLQEW DNAGKVARSH ILDKFIETNQ GKTAPELEQE FSQGASLFLV61RLTTSLRITY MTDSCLEKLL RSIGIFLSAV SSNRYLIEFL EVGGVLTLLE ILGLEKIKEE121AKKESVKLLQ VIANSGRTYK ELICESYGVR SIAEFLAKSK SEETQEEVQV LLDSLVHGNP181KYQNQVYKGL IALLPCESPK AQQLSLQTLR TAQPIIGTTH PSIVDCVLKV LGTMHLEVQY241EAIELIKDLV GYDVRQALLK GLVALLIPSV KEISKLQAKI LSDPSVLQLT PSLPMFLQQA301AAAKAIGVLA RNDMSIAEEL LYLRVVRGLM AAMGNTDHSN SQRLASLTLE CFVQMFPLVA361EHVRKCMGEE LYQLFLSNAE DLYMKIDSIQ ADILAANTVN VTKALCLHGS SYSMNTLYGS421RDSAQMAYLT HFEEDVESKETRTRPLEQKLISEEDLAANDILDYKDDDDKV
440 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
