C19orf35 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G103638-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human C19orf35 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G96178-20
Catalog Number: LS-G96178
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C19orf35 (NM_198532) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_198532
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSPEPPTEP PEPDNPTWST QPTYSNLGQI RAHLLPSKAC RLRTPGSLST NPEPLPPPLP61KKILTRTQSL PTRRTLHPSS IQVQPPRRPF LGSHSVDKSQ AAVGPACLPA ELTFGPADAP121LGLSLRDLHS PEAVHTALAA RQLQGLRTIY ARLRARLMGG HPGPCHPGHS FRLLDSSPCA181ESGDALYYRV VRAHEDAWHI LVAKVPKPGA DVPHPWGLEL QASLSPHFNL QGLCGLVPEG241TLPGAPWRGA VALAAEVPER TVAQWLAEAC TQPPEEFVWA VALLLLQLSA ALKFLEAWGA301ALVELRPENL LLVAPRGCAT TGPPRLLLTD FGRVCLQPPG PPGSPGPHAP QLGSLLRALL361SLAAPSTTPL AAGLELLAAQ LTRLRPSASR TRGALQALLW GPGPELRGRG APLGPWLRAL421GPWLRVRRGL LVLRLAERAA GGEAPSLEDW LCCEYLAEAT ESSMGQALAL LWDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
473 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
