C12orf66 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G106264-100
100 µg (1 mg/mL) / $504.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human C12orf66 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G94840-20
Catalog Number: LS-G94840
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C12orf66 (NM_152440) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C12orf66 | UPF0536 protein C12orf66
- Origin Species
- Human
- NCBI GenBank
- NM_152440
- SwissProt
- Q96MD2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGESIPLAAP VPVEQAVLET FFSHLGIFSY DKAKDNVEKE REANKSAGGS WLSLLAALAH61LAAAEKVYHS LTYLGQKLGG QSFFSRKDSI RTIYTSLHNE LKKVVTGRGA LGGTAPHVEE121LLSHLSEQLC FFVQARMEIA DFYEKMYTLS TQKFINAEEL VGLLDAIMKK YSSRFHHPIL181SPLESSFQLE VDVLCHLLKA QAQVSEWKFL PSLVNLHSAH TKLQTWGQIF EKQRETKKHL241FGGQSQKAVQ PPHLFLWLMK LKNMLLAKFS FYFHEALSRQ TTASEMKTLT AKANPDFFGK301ISSFIRKYDA ANVSLIFDNR GSESFQGHGY HHPHSYREAP KGVDQYPAVV SLPSDRPVMH361WPNVIMIMTD RTSDLNSLEK VVHFYDDKVQ STYFLTRPEP HFTIVIIFES KKSERDSHFI421SFLNEVSLAL KNPKVFASLK PGSKGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
445 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
