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Human BRD8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human BRD8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all BRD8 antibodies →
$220.00each
Catalog Number: LS-G80250-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human BRD8 (NM_139199) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BRD8 | p120 | TrCP120 | SMAP | Bromodomain containing 8
Origin Species
Human
NCBI GenBank
NM_006696
SwissProt
Q9H0E9

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
135.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATGTGKHKL LSTGPTEPWS IREKLCLASS VMRSGDQNWV SVSRAIKPFA EPGRPPDWFS
61QKHCASQYSE LLETTETPKR KRGEKGEVVE TVEDVIVRKL TAERVEELKK VIKETQERYR
121RLKRDAELIQ AGHMDSRLDE LCNDIATKKK LEEEEAEVKR KATDAAYQAR QAVKTPPRRL
181PTVMVRSPID SASPGGDYPL GDLTPTTMEE ATSGVNESEM AVASGHLNST GVLLEVGGVL
241PMIHGGEIQQ TPNTVAASPA ASGAPTLSRL LEAGPTQFTT PLASFTTVAS EPPVKLVPPP
301VESVSQATIV MMPALPAPSS APAVSTTESV APVSQPDNCV PMEAVGDPHT VTVSMDSSEI
361SMIINSIKEE CFRSGVAEAP VGSKAPSIDG KEELDLAEKM DIAVSYTGEE LDFETVGDII
421AIIEDKVDDH PEVLDVAAVE AALSFCEEND DPQSLPGPWE HPIQQERDKP VPLPAPEMTV
481KQERLDFEET ENKGIHELVD IREPSAEIKV EPAEPEPVIS GAEIVAGVVP ATSMEPPELR
541SQDLDEELGS TAAGEIVEAD VAIGKGDETP LTNVKTEASP ESMLSPSHGS NPIEDPLEAE
601TQHKFEMSDS LKEESGTIFG SQIKDAPGED EEEDGVSEAA SLEEPKEEDQ GEGYLSEMDN
661EPPVSESDDG FSIHNATLQS HTLADSIPSS PASSQFSVCS EDQEAIQAQK IWKKAIMLVW
721RAAANHRYAN VFLQPVTDDI APGYHSIVQR PMDLSTIKKN IENGLIRSTA EFQRDIMLMF
781QNAVMYNSSD HDVYHMAVEM QRDVLEQIQQ FLATQLIMQT SESGISAKSL RGRDSTRKQD
841ASEKDSVPMG SPAFLLSLFM GHEWVWLDSE QDHPNDSELS NDCRSLFSSW DSSLDLDVGN
901WRETEDPEAE ELEESSPERE PSELLVGDGG SEESQEAARK ASHQNLLHFL SEVAYLMEPL
961CISSNESSEG CCPPSGTRQE GREIKASEGE RELCRETEEL SAKGDPLVAE KPLGENGKPE
1021VASAPSVICT VQGLLTESEE GEAQQESKGE DQGEVYVSEM EDQPPSGECD DAFNIKETPL
1081VDTLFSHATS SKLTDLSQDD PVQDHLLFKK TLLPVWKMIA SHRFSSPFLK PVSERQAPGY
1141KDVVKRPMDL TSLKRNLSKG RIRTMAQFLR DLMLMFQNAV MYNDSDHHVY HMAVEMRREV
1201LEQIQVLNIW LDKRKGSSSL EGEPANPVDD GKPVF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1235 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.