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Human BICC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human BICC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all BICC1 antibodies →
$220.00each
Catalog Number: LS-G91900-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human BICC1 (NM_001080512) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BICC1 | CYSRD | Protein bicaudal C homolog 1 | Bic-C | BICC
Origin Species
Human
NCBI GenBank
AF224746
SwissProt
Q9IA00

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
104.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAQGEPGYL AAQSDPGSNS ERSTDSPVPG SEDDLVAGAT LHSPEWSEER FRVDRKKLEA
61MLQAAAEGKG RSGEDFFQKI MEETNTQIAW PSKLKIGAKS KKDPHIKVSG KKEDVKEAKE
121MIMSVLDTKS NRVTLKMDVS HTEHSHVIGK GGNNIKKVME ETGCHIHFPD SNRNNQAEKS
181NQVSIAGQPA GVESARVRIR ELLPLVLMFE LPIAGILQPV PDPNSPSIQH ISQTYNISVS
241FKQRSRMYGA TVIVRGSQNN TSAVKEGTAM LLEHLAGSLA SAIPVSTQLD IAAQHHLFMM
301GRNGSNIKHI MQRTGAQIHF PDPSNPQKKS TVYLQGTIES VCLARQYLMG CLPLVLMFDM
361KEEIEVDPQF IAQLMEQLDV FISIKPKPKQ PSKSVIVKSV ERNALNMYEA RKCLLGLESS
421GVTIATSPSP ASCPAGLACP SLDILASAGL GLTGLGLLGP TTLSLNTSTT PNSLLNALNS
481SVSPLQSPSS GTPSPTLWAP PLANTSSATG FSAIPHLMIP STAQATLTNI LLSGVPTYGH
541TAPSPPPGLT PVDVHINSMQ TEGKKISAAL NGHAQSPDIK YGAISTSSLG EKVLSANHGD
601PSIQTSGSEQ TSPKSSPTEG CNDAFVEVGM PRSPSHSGNA GDLKQMMCPS KVSCAKRQTV
661ELLQGTKNSH LHSTDRLLSD PELSATESPL ADKKAPGSER AAERAAAAQQ NSERAHLAPR
721SSYVNMQAFD YEQKKLLATK AMLKKPVVTE VRTPTNTWSG LGFSKSMPAE TIKELRRANH
781VSYKPTMTTT YEGSSMSLSR SNSREHLGGG SESDNWRDRN GIGPGSHSEF AASIGSPKRK
841QNKLTEHYLS SSNYMDCISS LTGSNGCNLN SSFKGSDLPE LFSKLGLGKY TDVFQQQEID
901LQTFLTLTDQ DLKELGITTF GARRKMLLAI SELNKNRRKL FESPNARTSF LEGGASGRLP
961RQYHSDIASV SGRW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

974 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.