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Human BCAP / PIK3AP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human BCAP / PIK3AP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all PIK3AP1 antibodies →
$220.00each
Catalog Number: LS-G94162-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human PIK3AP1 (NM_152309) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
B cell adaptor protein | BCAP | PIK3AP1 | RP11-34E5.3
Origin Species
Human
SwissProt
Q6ZUJ8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASGVPRGC DILIVYSPDA EEWCQYLQTL FLSSRQVRSQ KILTHRLGPE ASFSAEDLSL
61FLSTRCVVVL LSAELVQHFH KPSLLPLLQR AFHPPHRVVR LLCGVRDSEE FLDFFPDWAH
121WQELTCDDEP ETYVAAVKKA ISEDSGCDSV TDTEPEDEKV VSYSKQQNLP TVTSPGNLMV
181VQPDRIRCGA ETTVYVIVRC KLDDRVATEA EFSPEDSPSV RMEAKVENEY TISVKAPNLS
241SGNVSLKIYS GDLVVCETVI SYYTDMEEIG NLLSNAANPV EFMCQAFKIV PYNTETLDKL
301LTESLKNNIP ASGLHLFGIN QLEEEDMMTN QRDEELPTLL HFAAKYGLKN LTALLLTCPG
361ALQAYSVANK HGHYPNTIAE KHGFRDLRQF IDEYVETVDM LKSHIKEELM HGEEADAVYE
421SMAHLSTDLL MKCSLNPGCD EDLYESMAAF VPAATEDLYV EMLQASTSNP IPGDGFSRAT
481KDSMIRKFLE GNSMGMTNLE RDQCHLGQEE DVYHTVDDDE AFSVDLASRP PVPVPRPETT
541APGAHQLPDN EPYIFKVFAE KSQERPGNFY VSSESIRKGP PVRPWRDRPQ SSIYDPFAGM
601KTPGQRQLIT LQEQVKLGIV NVDEAVLHFK EWQLNQKKRS ESFRFQQENL KRLRDSITRR
661QREKQKSGKQ TDLEITVPIR HSQHLPAKVE FGVYESGPRK SVIPPRTELR RGDWKTDSTS
721STASSTSNRS STRSLLSVSS GMEGDNEDNE VPEVTRSRSP GPPQVDGTPT MSLERPPRVP
781PRAASQRPPT RETFHPPPPV PPRGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

805 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.