LSBio
Human BAF53B / ACTL6B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human BAF53B / ACTL6B Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ACTL6B antibodies →
$153.00each
Catalog Number: LS-G86394-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human BAF53b(ACTL6B) (NM_016188) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Actin-related protein Baf53b | ACTL6 | Actin-like 6B | ACTL6B | Actin-like 6 | ArpNalpha | BAF53B | BRG1-associated factor 53B | Actin-like protein 6B | HArpN alpha
Origin Species
Human
NCBI GenBank
NM_016188
SwissProt
O94805

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGGVYGGDE VGALVFDIGS FSVRAGYAGE DCPKADFPTT VGLLAAEEGG GLELEGDKEK
61KGKIFHIDTN ALHVPRDGAE VMSPLKNGMI EDWECFRAIL DHTYSKHVKS EPNLHPVLMS
121EAPWNTRAKR EKLTELMFEQ YNIPAFFLCK TAVLTAFANG RSTGLVLDSG ATHTTAIPVH
181DGYVLQQGIV KSPLAGDFIS MQCRELFQEM AIDIIPPYMI AAKEPVREGA PPNWKKKEKL
241PQVSKSWHNY MCNEVIQDFQ ASVLQVSDSP YDEQVAAQMP TVHYEMPNGY NTDYGAERLR
301IPEGLFDPSN VKGLSGNTML GVGHVVTTSI GMCDIDIRPG LYGSVIVTGG NTLLQGFTDR
361LNRELSQKTP PSMRLKLIAS NSTMERKFSP WIGGSILASL GTFQQMWISK QEYEEGGKQC
421VERKCP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

426 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.