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Human AZIN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human AZIN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all AZIN1 antibodies →
$153.00each
Catalog Number: LS-G81090-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Antizyme inhibitor 1(AZIN1) (NM_015878) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AZIN1 | Antizyme inhibitor | ODC1L | OAZI | Antizyme inhibitor 1 | AZI | OAZIN
Origin Species
Human
NCBI GenBank
NM_015878
SwissProt
O14977

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MKGFIDDANY SVGLLDEGTN LGNVIDNYVY EHTLTGKNAF FVGDLGKIVK KHSQWQNVVA
61QIKPFYTVKC NSAPAVLEIL AALGTGFACS SKNEMALVQE LGVPPENIIY ISPCKQVSQI
121KYAAKVGVNI LTCDNEIELK KIARNHPNAK VLLHIATEDN IGGEEGNMKF GTTLKNCRHL
181LECAKELDVQ IIGVKFHVSS ACKESQVYVH ALSDARCVFD MAGEIGFTMN MLDIGGGFTG
241TEFQLEEVNH VISPLLDIYF PEGSGVKIIS EPGSYYVSSA FTLAVNIIAK KVVENDKFPS
301GVEKTGSDEP AFMYYMNDGV YGSFASKLSE DLNTIPEVHK KYKEDEPLFT SSLWGPSCDE
361LDQIVESCLL PELNVGDWLI FDNMGADSFH EPSAFNDFQR PAIYYMMSFS DWYEMQDAGI
421TSDSMMKNFF FVPSCIQLSQ EDSFSAEA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

448 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.