LSBio
Human AVPR1A / V1a Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human AVPR1A / V1a Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all AVPR1A antibodies →
$153.00each
Catalog Number: LS-G79541-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AVPR1A (NM_000706) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AVPR V1a | AVPR1A | V1a receptor | AVPR1 | V1a vasopressin receptor | Vasopressin V1a receptor | V1aR
Origin Species
Human
NCBI GenBank
NM_000706
SwissProt
P37288

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MRLSAGPDAG PSGNSSPWWP LATGAGNTSR EAEALGEGNG PPRDVRNEEL AKLEIAVLAV
61TFAVAVLGNS SVLLALHRTP RKTSRMHLFI RHLSLADLAV AFLQVLPQMC WDITYRFRGP
121DWLCRVVKHL QVFGMFASAY MLVVMTADRY IAVCHPLKTL QQPARRSRLM IAAAWVLSFV
181LSTPQYFVFS MIEVNNVTKA RDCWATFIQP WGSRAYVTWM TGGIFVAPVV ILGTCYGFIC
241YNIWCNVRGK TASRQSKGAE QAGVAFQKGF LLAPCVSSVK SISRAKIRTV KMTFVIVTAY
301IVCWAPFFII QMWSVWDPMS VWTESENPTI TITALLGSLN SCCNPWIYMF FSGHLLQDCV
361QSFPCCQNMK EKFNKEDTDS MSRRQTFYSN NRSPTNSTGM WKDSPKSSKS IKFIPVST
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

418 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.