LSBio
Human ATP6V1H Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ATP6V1H Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ATP6V1H antibodies →
$220.00each
Catalog Number: LS-G82805-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ATP6V1H (NM_213619) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATP6V1H | Nef-binding protein 1 | SFD | V-ATPase 50/57 kDa subunits | V-ATPase subunit H | Vacuolar ATPase subunit H | Protein VMA13 homolog | SFDalpha | CGI-11 | MSTP042 | NBP1 | SFDbeta | V-type proton ATPase subunit H | Vacuolar proton pump subunit H | VMA13
Origin Species
Human
NCBI GenBank
NM_015941
SwissProt
Q9UI12

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54 kda
Protein Type
Lysate
Amino Acid Sequence
1MTKMDIRGAV DAAVPTNIIA AKAAEVRANK VNWQSYLQGQ MISAEDCEFI QRFEMKRSPE
61EKQEMLQTEG SQCAKTFINL MTHICKEQTV QYILTMVDDM LQENHQRVSI FFDYARCSKN
121TAWPYFLPML NRQDPFTVHM AARIIAKLAA WGKELMEGSD LNYYFNWIKT QLSSQSSQYV
181QCVAGCLQLM LRVNEYRFAW VEADGVNCIM GVLSNKCGFQ LQYQMIFSIW LLAFSPQMCE
241HLRRYNIIPV LSDILQESVK EKVTRIILAA FRNFLEKSTE RETRQEYALA MIQCKVLKQL
301ENLEQQKYDD EDISEDIKFL LEKLGESVQD LSSFDEYSSE LKSGRLEWSP VHKSEKFWRE
361NAVRLNEKNY ELLKILTKLL EVSDDPQVLA VAAHDVGEYV RHYPRGKRVI EQLGGKQLVM
421NHMHHEDQQV RYNALLAVQK LMVHNWEYLG KQLQSEQPQT AAARS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

465 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.