Human ATHL1 Protein (Recombinant His, N-Terminal)
Catalog Number: LS-G144526-50
50 µg / $428.00
Expression System Escherichia coli (Prokaryotic)
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ATHL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G83100-20
Catalog Number: LS-G83100
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ATHL1(PGGHG) (NM_025092) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Acid trehalase-like protein 1 | ATHL1
- Origin Species
- Human
- NCBI GenBank
- NM_025092
- SwissProt
- Q32M88
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLWTPAPPDL TLGEGEEART WDFLTAVGGS QAEAQACLTE ALQLQARGAL YTAHAQAWAQ61LWVECGLDVV GPLQLRQALR GSLYYLLSAL PQPKAPGYIC HGLSPGGLSN GSREECYWGH121VFWDQDLWMF PSILMFHPEA ARAILEYRIR TLDGALENAQ NLGYQGAKFA WESADSGLEV181CPEDIYGVQE VHVNGAVVLA FELYYHTTQD LQLFREAGGW DVVRAVAEFW CSRVEWSPRE241EKYHLRGVMS PDEYHSGVNN SVYTNVLVQN SLRFAAALAQ DLGLPIPSQW LAVADKIKVP301FDVEQNFHPE FDGYEPGEVV KQADVVLLGY PVPFSLSPDV RRKNLEIYEA VTSPQGPAMT361WSMFAVGWME LKDAVRARGL LDRSFANMAE PFKVWTENAD GSGAVNFLTG MGGFLQAVVF421GCTGFRVTRA GVTFDPVCLS GISRVSVSGI FYQGNKLNFS FSEDSVTVEV TARAGPWAPH481LEAELWPSQS RLSLLPGHKV SFPRSAGRIQ MSPPKLPGSS SSEFPGRTFS DVRDPLQSPL541WVTLGSSSPT ESLTVDPASETRTRPLEQKLISEEDLAANDILDYKDDDDKV
560 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.