LSBio
Human ATF7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ATF7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ATF7 antibodies →
$153.00each
Catalog Number: LS-G79213-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ATF7 (NM_001130059) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATFA | Transcription factor ATF-A | ATF7
Origin Species
Human
NCBI GenBank
NM_006856
SwissProt
P17544

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDDRPFVCN APGCGQRFTN EDHLAVHKHK HEMTLKFGPA RTDSVIIADQ TPTPTRFLKN
61CEEVGLFNEL ASSFEHEFKK AADEDEKKAR SRTVAKKLVA AAGPLDMSLP STPDIKIKEE
121EPVEVDSSPP DSPASSPCSP PLKEKEVTPK PVLISTPTPT IVRPGSLPLH LGYDPLHPTL
181PSPTSVITQA PPSNRQMGSP TGSLPLVMHL ANGQTMPVLP GPPVQMPSVI SLARPVSMVP
241NIPGIPGPPV NSSGSISPSG HPIPSEAKMR LKATLTHQVS SINGGCGMVV GTASTMVTAR
301PEQSQILIQH PDAPSPAQPQ VSPAQPTPST GGRRRRTVDE DPDERRQRFL ERNRAAASRC
361RQKRKLWVSS LEKKAEELTS QNIQLSNEVT LLRNEVAQLK QLLLAHKDCP VTALQKKTQG
421YLESPKESSE PTGSPAPVIQ HSSATAPSNG LSVRSAAEAV ATSVLTQMAS QRTELSMPIQ
481SHVIMTPQSQ SAGR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

494 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.