LSBio
Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 8 (AGAP8) Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 8 (AGAP8) Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
$220.00each
Catalog Number: LS-G94787-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AGAP8 (NM_001077686) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
SwissProt
Q5SRD3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGNILTCRVH PSVSLEFDQQ QGSVCPSESE IYEAGAGDRM AGAPMAAAVQ PAEVTVEVGE
61DLHMHHVRDR EMPEALEFNP SANPEASTIF QRNSQTDVVE IRRSNCTNHV STVRFSQQYS
121LCSTIFLDDS TAIQHYLTMT IISVTLEIPH HITQRDADRS LSIPDEQLHS FAVSTVHIMK
181KRNGGGSLNN YSSSIPSTPS TSQEDPQFSV PPTANTPTPV CKRSMRWSNL FTSEKGSDPD
241KERKAPENHA DTIGSGRAIP IKQGMLLKRS GKWLKTWKKK YVTLCSNGVL TYYSSLGDYM
301KNIHKKEIDL QTSTIKVPGK WPSLATSACT PISSSKSNGL SKDMDTGLGD SICFSPSISS
361TTSPKLNPPP SPHANKKKHL KKKSTNNFMI VSATGQTWHF EATTYEERDA WVQAIQSQIL
421ASLQSCESSK SKSQLTSQSE AMALQSIQNM RGNAHCVDCE TQNPKWASLN LGVLMCIECS
481GIHHSLGTRL SRVRSLELDD WPVELRKVMS SIGNDLANSI WEGSSQGQTK PSEKSTREEK
541ERWIRSKYEE KLFLAPLPCT ELSLGQQLLW ATADEDLQTA ILLLAHGSRE EVNETCGEGD
601GCTALHLACR KGNVVLAQLL IWYGVDVMAR DAHGNTALTY ARQASSQECI NVLLQYGCPD
661ECV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

663 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.