API5 Antibody
Catalog Number: LS-C406134
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human API5 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G85750-20
Catalog Number: LS-G85750
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Apoptosis inhibitor 5(API5) (NM_001142931) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AAC11 | AAC-11 | API5 | Antiapoptosis clone 11 protein | API-5 | Apoptosis inhibitor 5 | FIF | API5L1 | Protein XAGL
- Origin Species
- Human
- NCBI GenBank
- NM_006595
- SwissProt
- Q9BZZ5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPTVEELYRN YGILADATEQ VGQIRRQAIK ELPQFATGEN LPRVADILTQ LLQTDDSAEF61NLVNNALLSI FKMDAKGTLG GLFSQILQGE DIVRERAIKF LSTKLKTLPD EVLTKEVEEL121ILTESKKVLE DVTGEEFVLF MKILSGLKSL QTVSGRQQLV ELVAEQADLE QTFNPSDPDC181VDRLLQCTRQ AVPLFSKNVH STRFVTYFCE QVLPNLGTLT TPVEGLDIQL EVLKLLAEMS241SFCGDMEKLE TNLRKLFDKL LEYMPLPPEE AENGENAGNE EPKLQFSYVE CLLYSFHQLG301RKLPDFLTAK LNAEKLKDFK IRLQYFARGL QVYIRQLRLA LQGKTGEALK TEENKIKVVA361LKITNNINVL IKDLFHIPPS YKSTVTLSWK PVQKVEIGQK RASEDTTSGS PPKKSSAGPK421RDARQIYNPP SGKYSSNLGN FNYERSLQGKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
450 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
