LSBio
Human AOAH / Acyloxyacyl Hydrolase Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human AOAH / Acyloxyacyl Hydrolase Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all AOAH antibodies →
$153.00each
Catalog Number: LS-G74311-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Acyloxyacyl Hydrolase(AOAH) (NM_001177507) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Acyloxyacyl hydrolase | AOAH
Origin Species
Human
NCBI GenBank
NM_001637
SwissProt
P28039

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQSPWKILTV APLFLLLSLQ SSASPANDDQ SRPSLSNGHT CVEKLFLKTT CYLVIDKFGS
61DIIKLLSADM NADVVCHTLE FCKQNTGQPL CHLYPLPKET WKFTLQKARQ IVKKSPILKY
121SRSGSDICSL PVLAKICQKI KLAMEQSVPF KDVDSDKYSV FPTLRGYHWR GRDCNDSDES
181VYPGRRPNNW DVHQDSNCNG IWGVDPKDGV PYEKKFCEGS QPRGIILLGD SAGAHFHISP
241EWITASQMSL NSFINLPTAL TNELDWPQLS GATGFLDSTV GIKEKSIYLR LWKRNHCNHR
301DYQNISRNGA SSRNLKKFIE SLSRNKVLDY PAIVIYAMIG NDVCSGKSDP VPAMTTPEKL
361YSNVMQTLKH LNSHLPNGSH VILYGLPDGT FLWDNLHNRY HPLGQLNKDM TYAQLYSFLN
421CLQVSPCHGW MSSNKTLRTL TSERAEQLSN TLKKIAASEK FTNFNLFYMD FAFHEIIQEW
481QKRGGQPWQL IEPVDGFHPN EVALLLLADH FWKKVQLQWP QILGKENPFN PQIKQVFGDQ
541GGH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

543 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.