Human ANKRD34A Protein (Recombinant Myc-DDK (Flag))
Catalog Number: LS-G73702-20
20 µg / $1130.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ANKRD34A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95945-20
Catalog Number: LS-G95945
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD34A (NM_001039888) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001039888
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLHTEGHALL RAVGQGKLRL ARLLLEGGAY VNEGDAQGET ALMAACRARY DDPQNKARMV61RYLLEQGADP NIADRLGRTA LMHACAGGGG AAVASLLLAH GADPSVRDHA GASALVHALD121RGDRETLATL LDACKAKGTE VIIITTDTSP SGTKKTRQYL NSPPSPGVED PAPASPSPGF181CTSPSEIQLQ TAGGGGRGML SPRAQEEEEK RDVFEFPLPK PPDDPSPSEP LPKPPRHPPK241PLKRLNSEPW GLVAPPQPVP PTEGRPGIER LTAEFNGLTL TGRPRLSRRH STEGPEDPPP301WAEKVTSGGP LSRRNTAPEA QESGPPSGLR QKLSRMEPVE LDTPGHLCPD SPESSRLSLE361RRRYSASPLT LPPAGSAPSP RQSQESLPGA VSPLSGRRRS PGLLERRGSG TLLLDHISQT421RPGFLPPLNV SPHPPIPDIR PQPGGRAPSL PAPPYAGAPG SPRTKRKLVR RHSMQTEQIR481LLGGFQSLGG PGEPGRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
496 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
