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Human ANKLE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ANKLE1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ANKLE1 antibodies →
$220.00each
Catalog Number: LS-G94478-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ANKLE1 (NM_152363) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ankyrin repeat domain 41 | ANKLE1 | LEM domain containing 6 | LEM3 | LEMD6 | ANKRD41 | LEM-domain containing 3
Origin Species
Human
SwissProt
Q8NAG6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCSEARLARR LRDALREEEP WAVEELLRCG ADPNLVLEDG AAAVHLAAGA RHPRGLRCLG
61ALLRQGGDPN ARSVEALTPL HVAAAWGCRR GLELLLSQGA DPALRDQDGL RPLDLALQQG
121HLECARVLQD LDTRTRTRTR IGAETQEPEP APGTPGLSGP TDETLDSIAL QKQPCRGDNR
181DIGLEADPGP PSLPVPLETV DKHGSSASPP GHWDYSSDAS FVTAVEVSGA EDPASDTPPW
241AGSLPPTRQG LLHVVHANQR VPRSQGTEAE LNARLQALTL TPPNAAGFQS SPSSMPLLDR
301SPAHSPPRTP TPGASDCHCL WEHQTSIDSD MATLWLTEDE ASSTGGREPV GPCRHLPVST
361VSDLELLKGL RALGENPHPI TPFTRQLYHQ QLEEAQIAPG PEFSGHSLEL AAALRTGCIP
421DVQADEDALA QQFEQPDPAR RWREGVVKSS FTYLLLDPRE TQDLPARAFS LTPAERLQTF
481IRAIFYVGKG TRARPYVHLW EALGHHGRSR KQPHQACPKV RQILDIWASG CGVVSLHCFQ
541HVVAVEAYTR EACIVEALGI QTLTNQKQGH CYGVVAGWPP ARRRRLGVHL LHRALLVFLA
601EGERQLHPQD IQARG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

615 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.