ANKEF1 / ANKRD5 Antibody (aa721-749, APC)
Catalog Number: LS-C221430-200
200 µL / $972.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human ANKEF1 / ANKRD5 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G87786-20
Catalog Number: LS-G87786
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD5(ANKEF1) (NM_198798) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANKRD5 | ANKEF1 | DJ839B4.6
- Origin Species
- Human
- NCBI GenBank
- NM_022096
- SwissProt
- Q9NU02
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALADKGLEN LQIYKVLQCV RNKDKKQIEK LTKLGYPELI NYTEPINGLS ALHLASVSND61IDMVSFLLDL GAHPDVQDRM GCTPTMRAAE LGHELSMEIL AKAKADMTIV DNEGKGVLFY121CILPTKRHYR CALIALEHGA DVNNSTYEGK PIFLRACEDA HDVKDVCLTF LEKGANPNAI181NSSTGRTALM EASREGVVEI VRGILERGGE VNAFDNDRHH AAHFAAKGGF FDILKLLFAY241NGDVGLISIN GNTPLHYAAM GGFADCCKYI AQRGCDLKWK NLDHKTPRAV AKEGGFKAAS301KEIRRAERIA NKLARPGAKN PNPLWALRLH DWSVEREAFL REAFAVLDRG DGSISKNDFV361MVLEERQDYA SSEQLAAIAH LHEKTRGGGV NINEFFKGTR YLNKSFVLGS YGPKKKEKGM421GKKGKKGKFV LPLPICVIPE YAFPRRQDGG PPYYMIETYK NVTDSSRFNR DHPPEHPIQD481DSVWYIDDSE KVFSNINIIT KAGDLASLKK AFESGIPVDM KDNYYKTPLM TACASGNIDV541VKFLLEKGAN VNATDNFLWT PLHFACHAGQ QDIVELLVES GALIDAASIN NSTPLNRAIE601SCRLDTVKYL LDIGAKFQLE NRKGHSAMDV AKAYADYRII DLIKEKLDNL PKPAENQKLK661GKTPPILKTE GPEIKKEEEL LSSIYGVPTT SEGKKVQKGN VVHLNSLITS GYTKKVDITF721IPRRIWSPEA TTAELIRKRE LRRERFTHEV DFDDFMMPFQ KNITEKSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
766 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
