LSBio
Human ALOX15B / 15-LOX-2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ALOX15B / 15-LOX-2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ALOX15B antibodies →
$220.00each
Catalog Number: LS-G81817-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human 15 Lipoxygenase 2(ALOX15B) (NM_001039131) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
15S-lipoxygenase | 15-lipoxygenase 2 | 15-LOX-2 | 15-LOX-B | Arachidonate 15-lipoxygenase B | ALOX15B | Arachidonate 15-lipoxygenase 2
Origin Species
Human
NCBI GenBank
NM_001141
SwissProt
O15296

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEFRVRVST GEAFGAGTWD KVSVSIVGTR GESPPLPLDN LGKEFTAGAE EDFQVTLPED
61VGRVLLLRVH KAPPVLPLLG PLAPDAWFCR WFQLTPPRGG HLLFPCYQWL EGAGTLVLQE
121GTAKVSWADH HPVLQQQRQE ELQARQEMYQ WKAYNPGWPH CLDEKTVEDL ELNIKYSTAK
181NANFYLQAGS AFAEMKIKGL LDRKGLWRSL NEMKRIFNFR RTPAAEHAFE HWQEDAFFAS
241QFLNGLNPVL IRRCHYLPKN FPVTDAMVAS VLGPGTSLQA ELEKGSLFLV DHGILSGIQT
301NVINGKPQFS AAPMTLLYQS PGCGPLLPLA IQLSQTPGPN SPIFLPTDDK WDWLLAKTWV
361RNAEFSFHEA LTHLLHSHLL PEVFTLATLR QLPHCHPLFK STGIGIEGFS ELIQRNMKQL
421NYSLLCLPED IRTRGVEDIP GYYYRDDGMQ IWGIPSSLET REALVQYVTM VIFTCSAKHA
481AVSAGQFDSC AWMPNLPPSM QLPPPTSKGL ATCEGFIATL PPVNATCDVI LALWLLSKEP
541GDQRPLGTYP DEHFTEEAPR RSIATFQSRL AQISRGIQER NQGLVLPYTY LDPPLIENSV
601SI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

602 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.