AIRE Antibody
Catalog Number: LS-C783525-25
25 µg (0.5 mg/mL) / $287.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human AIRE Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G79830-20
Catalog Number: LS-G79830
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AIRE (NM_000658) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APSI | AIRE | AIRE1 | Autoimmune regulator | APECED | APS1 | APECED protein | PGA1
- Origin Species
- Human
- NCBI GenBank
- NM_000383
- SwissProt
- O43918
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MWLVYSSGAP GTQQPARNRV FFPIGMAPGG VCWRPDGWGT GGQGRISGPG SMGAGQRLGS61SGTQRCCWGS CFGKEVALRR VLHPSPVCMG VSCLCQKNED ECAVCRDGGE LICCDGCPRA121FHLACLSPPL REIPSGTWRC SSCLQATVQE VQPRAEEPRP QEPPVETPLP PGLRSAGEEV181RGPPGEPLAG MDTTLVYKHL PAPPSAAPLP GLDSSALHPL LCVGPEGQQN LAPGARCGVC241GDGTDVLRCT HCAAAFHWRC HFPAGTSRPG TGLRCRSCSG DVTPAPVEGV LAPSPARLAP301GPAKDDTASH EPALHRDDLE SLLSEHTFDG ILQWAIQSMA RPAAPFPSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
348 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
