LSBio
Human AGFG2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human AGFG2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all AGFG2 antibodies →
$153.00each
Catalog Number: LS-G89472-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human AGFG2 (NM_006076) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AGFG2 | ArfGAP with FG repeats 2 | HIV-1 Rev binding protein-like | HRBL | Nucleoporin | RAB-R | RABR
Origin Species
Human
NCBI GenBank
NM_006076
SwissProt
O95081

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
48.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVMAAKKGPG PGGGVSGGKA EAEAASEVWC RRVRELGGCS QAGNRHCFEC AQRGVTYVDI
61TVGSFVCTTC SGLLRGLNPP HRVKSISMTT FTEPEVVFLQ SRGNEVCRKI WLGLFDARTS
121LVPDSRDPQK VKEFLQEKYE KKRWYVPPDQ VKGPTYTKGS ASTPVQGSIP EGKPLRTLLG
181DPAPSLSVAA STSSQPVSQS HARTSQARST QPPPHSSVKK ASTDLLADIG GDPFAAPQMA
241PAFAAFPAFG GQTPSQGGFA NFDAFSSGPS SSVFGSLPPA GQASFQAQPT PAGSSQGTPF
301GATPLAPASQ PNSLADVGSF LGPGVPAAGV PSSLFGMAGQ VPPLQSVTTG GGGGSSTGLA
361FGAFTNPFTA PAAQSPLPST NPFQPNGLAP GPGFGMSSAG PGFPQAVPPT GAFASSFPAP
421LFPPQTPLVQ QQNGSSFGDL GSAKLGQRPL SQPAGISTNP FMTGPSSSPF ASKPPTTNPF
481L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

481 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.