LSBio
Human ADGB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ADGB Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ADGB antibodies →
$220.00each
Catalog Number: LS-G83843-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ADGB (NM_024694) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_024694
SwissProt
Q8N7X0

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
189.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASKQTKKKE VHRINSAHGS DKSKDFYPFG SNVQSGSTEQ KKGKFPLWPE WSEADINSEK
61WDAGKGAKEK DKTGKSPVFH FFEDPEGKIE LPPSLKIYSW KRPQDILFSQ TPVVVKNEIT
121FDLFSANEHL LCSELMRWII SEIYAVWKIF NGGILSNYFK GTSGEPPLLP WKPWEHIYSL
181CKAVKGHMPL FNSYGKYVVK LYWMGCWRKI TIDDFLPFDE DNNLLLPATT YEFELWPMLL
241SKAIIKLANI DIHVADRREL GEFTVIHALT GWLPEVISLH PGYMDKVWEL LKEILPEFKL
301SDEASSESKI AVLDSKLKEP GKEGKEGKEI KDGKEVKDVK EFKPESSLTT LKAPEKSDKV
361PKEKADARDI GKKRSKDGEK EKFKFSLHGS RPSSEVQYSV QSLSDCSSAI QTSHMVVYAT
421FTPLYLFENK IFSLEKMADS AEKLREYGLS HICSHPVLVT RSRSCPLVAP PKPPPLPPWK
481LIRQKKETVI TDEAQELIVK KPERFLEISS PFLNYRMTPF TIPTEMHFVR SLIKKGIPPG
541SDLPSVSETD ETATHSQTDL SQITKATSQG NTASQVILGK GTDEQTDFGL GDAHQSDGLN
601LEREIVSQTT ATQEKSQEEL PTTNNSVSKE IWLDFEDFCV CFQNIYIFHK PSSYCLNFQK
661SEFKFSEERV SYYLFVDSLK PIELLVCFSA LVRWGEYGAL TKDSPPIEPG LLTAETFSWK
721SLKPGSLVLK IHTYATKATV VRLPVGRHML LFNAYSPVGH SIHICSMVSF VIGDEHVVLP
781NFEPESCRFT EQSLLIMKAI GNVIANFKDK GKLSAALKDL QTAHYPVPFH DKELTAQHFR
841VFHLSLWRLM KKVQITKPPP NFKFAFRAMV LDLELLNSSL EEVSLVEWLD VKYCMPTSDK
901EYSAEEVAAA IKIQAMWRGT YVRLLMKARI PDTKENISVA DTLQKVWAVL EMNLEQYAVS
961LLRLMFKSKC KSLESYPCYQ DEETKIAFAD YTVTYQEQPP NSWFIVFRET FLVHQDMILV
1021PKVYTTLPIC ILHIVNNDTM EQVPKVFQKV VPYLYTKNKK GYTFVAEAFT GDTYVAASRW
1081KLRLIGSSAP LPCLSRDSPC NSFAIKEIRD YYIPNDKKIL FRYSVKVLTP QPATIQVRTS
1141KPDAFIKLQV LENEETMVSS TGKGQAIIPA FHFLKSEKGL SSQSSKHILS FHSASKKEQE
1201VYVKKKAAQG IQKSPKGRAV SAIQDIGLPL VEEETTSTPT REDSSSTPLQ NYKYIIQCSV
1261LYNSWPLTES QLTFVQALKD LKKSNTKAYG ERHEELINLG SPDSHTISEG QKSSVTSKTT
1321RKGKEKSSEK EKTAKEKQAP RFEPQISTVH PQQEDPNKPY WILRLVTEHN ESELFEVKKD
1381TERADEIRAM KQAWETTEPG RAIKASQARL HYLSGFIKKT SDAESPPISE SQTKPKEEVE
1441TAARGVKEPN SKNSAGSESK EMTQTGSGSA VWKKWQLTKG LRDVAKSTSS ESGGVSSPGK
1501EEREQSTRKE NIQTGPRTRS PTILETSPRL IRKALEFMDL SQYVRKTDTD PLLQTDELNQ
1561QQAMQKAEEI HQFRQHRTRV LSIRNIDQEE RLKLKDEVLD MYKEMQDSLD EARQKIFDIR
1621EEYRNKLLEA EHLKLETLAA QEAAMKLETE KMTPAPDTQK KKKGKKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1667 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.