Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ACSS2 / ACAS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression System	Human Embryonic Kidney 293T
Format:	Lyophilized

$153.00each

Catalog Number: LS-G86624-20

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Overview

Description: This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Acetyl CoA synthetase(ACSS2) (NM_018677) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category: Proteins & Peptides > Proteins > Lysates
Alternative Names: Acetate-CoA ligase | Acetyl-CoA synthetase | ACSS2 | Acetate--CoA ligase | ACS | ACSA | ACAS2 | ACECS | DJ1161H23.1 | Acetate thiokinase | Acyl-activating enzyme
Origin Species: Human
NCBI GenBank: NM_018677
SwissProt: Q9NR19

Protein

Expression System: Human Embryonic Kidney 293T
Fusion Tag: c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight: 78.4 kDa
Protein Type: Lysate
Amino Acid Sequence

Format

Purity: Unpurified
Conjugate/Label: Unconjugated
Endotoxin Level: Not Tested
Format: Lyophilized
Formulation: Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4