Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HTR5A / 5-HT5A Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101857-100
Catalog Number: LS-G101857
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human 5HT5A receptor(HTR5A) (NM_024012), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 5-HT-5 | 5-HT-5A | 5-HT5A | 5-HT5a receptor | 5-hydroxytryptamine5a | HTR5A | Serotonin 5-HT5a receptor | Serotonin 5a receptor | Serotonin receptor 5A
- Origin Species
- Human
- NCBI GenBank
- NM_024012
- SwissProt
- P47898
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDLPVNLTSF SLSTPSPLET NHSLGKDDLR PSSPLLSVFG VLILTLLGFL VAATFAWNLL61VLATILRVRT FHRVPHNLVA SMAVSDVLVA ALVMPLSLVH ELSGRRWQLG RRLCQLWIAC121DVLCCTASIW NVTAIALDRY WSITRHMEYT LRTRKCVSNV MIALTWALSA VISLAPLLFG181WGETYSEGSE ECQVSREPSY AVFSTVGAFY LPLCVVLFVY WKIYKAAKFR VGSRKTNSVS241PISEAVEVKD SAKQPQMVFT VRHATVTFQP EGDTWREQKE QRAALMVGIL IGVFVLCWIP301FFLTELISPL CSCDIPAIWK SIFLWLGYSN SFFNPLIYTA FNKNYNSAFK NFFSRQHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
357 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.