Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HTR3D / 5HT3D Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128151-100
Catalog Number: LS-G128151
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HTR3D (NM_001163646), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 5-HT3D | 5HT3D | 5-HT3-D | HTR3D | Serotonin 5-HT-3D receptor | Serotonin receptor 3D
- Origin Species
- Human
- SwissProt
- Q70Z44
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQKHSPGPPA LALLSQSLLT TGNGDTLIIN CPGFGQHRVD PAAFQAVFDR KAIGPVTNYS61VATHVNISFT LSAIWNCYSR IHTFNCHHAR PWHNQFVQWN PDECGGIKKS GMATENLWLS121DVFIEESVDQ TPAGLMASMS IVKATSNTIS QCGWSASANW TPSISPSMDR ARAWRRMSRS181FQIHHRTSFR TRREWVLLGI QKRTIKVTVA TNQYEQAIFH VAIRRRCRPS PYVVNFLVPS241GILIAIDALS FYLPLESGNC APFKMTVLLG YSVFLLMMND LLPATSTSSH ASLVAPLALM301QTPLPAGVYF ALCLSLMVGS LLETIFITHL LHVATTQPLP LPRWLHSLLL HCTGQGRCCP361TAPQKGNKGP GLTPTHLPGV KEPEVSAGQM PGPGEAELTG GSEWTRAQRE HEAQKQHSVE421LWVQFSHAMD ALLFRLYLLF MASSIITVIC LWNTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
454 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.