Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HTR3C / 5-HT3C Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G107640-100
Catalog Number: LS-G107640
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HTR3C (NM_130770), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 5-HT3-C | 5-HT3C | HTR3C | Serotonin receptor 3C
- Origin Species
- Human
- NCBI GenBank
- NM_130770
- SwissProt
- Q8WXA8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGGWPARQS ALLCLTVSLL LQGRGDAFTI NCSGFDQHGV DPAVFQAVFD RKAFRPFTNY61SIPTRVNISF TLSAILGVDA QLQLLTSFLW MDLVWDNPFI NWNPKECVGI NKLTVLAENL121WLPDIFIVES MDVDQTPSGL TAYISSEGRI KYDKPMRVTS ICNLDIFYFP FDQQNCTFTF181SSFLYTVDSM LLGMDKEVWE ITDTSRKVIQ TQGEWELLGI NKATPKMSMG NNLYDQIMFY241VAIRRRPSLY IINLLVPSSF LVAIDALSFY LPAESENRAP FKITLLLGYN VFLLMMNDLL301PASGTPLISV YFALCLSLMV VSLLETVFIT YLLHVATTQP PPMPRWLHSL LLHCTSPGRC361CPTAPQKGNK GLGLTLTHLP GPKEPGELAG KKLGPRETEP DGGSGWTKTQ LMELWVQFSH421AMDTLLFRLY LLFMASSILT VIVLWNTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
447 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.