Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HSPG2 / Perlecan Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G115909-100
Catalog Number: LS-G115909
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Heparan Sulfate Proteoglycan 2(HSPG2) (NM_005529), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Endorepellin (domain V region) | HSPG | HSPG2 | Heparan sulfate proteoglycan 2 | Perlecan | Perlecan proteoglycan | SJA | SJS1 | PRCAN | SJS
- Origin Species
- Human
- NCBI GenBank
- NM_005529
- SwissProt
- P98160
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 468.91 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGWRAAGALL LALLLHGRLL AVTHGLRAYD GLSLPEDIET VTASQMRWTH SYLSDDEDML61ADSISGDDLG SGDLGSGDFQ MVYFRALVNF TRSIEYSPQL EDAGSREFRE VSEAVVDTLE121SEYLKIPGDQ VVSVVFIKEL DGWVFVELDV GSEGNADGAQ IQEMLLRVIS SGSVASYVTS181PQGFQFRRLG TVPQFPRACT EAEFACHSYN ECVALEYRCD RRPDCRDMSD ELNCEEPVLG241ISPTFSLLVE TTSLPPRPET TIMRQPPVTH APQPLLPGSV RPLPCGPQEA ACRNGHCIPR301DYLCDGQEDC EDGSDELDCG PPPPCEPNEF PCGNGHCALK LWRCDGDFDC EDRTDEANCP361TKRPEEVCGP TQFRCVSTNM CIPASFHCDE ESDCPDRSDE FGCMPPQVVT PPRESIQASR421GQTVTFTCVA IGVPTPIINW RLNWGHIPSH PRVTVTSEGG RGTLIIRDVK ESDQGAYTCE481AMNARGMVFG IPDGVLELVP QRAGPCPDGH FYLEHSAACL PCFCFGITSV CQSTRRFRDQ541IRLRFDQPDD FKGVNVTMPA QPGTPPLSST QLQIDPSLHE FQLVDLSRRF LVHDSFWALP601EQFLGNKVDS YGGSLRYNVR YELARGMLEP VQRPDVVLVG AGYRLLSRGH TPTQPGALNQ661RQVQFSEEHW VHESGRPVQR AELLQVLQSL EAVLIQTVYN TKMASVGLSD IAMDTTVTHA721TSHGRAHSVE ECRCPIGYSG LSCESCDAHF TRVPGGPYLG TCSGCSCNGH ASSCDPVYGH781CLNCQHNTEG PQCNKCKAGF FGDAMKATAT SCRPCPCPYI DASRRFSDTC FLDTDGQATC841DACAPGYTGR RCESCAPGYE GNPIQPGGKC RPVNQEIVRC DERGSMGTSG EACRCKNNVV901GRLCNECADG SFHLSTRNPD GCLKCFCMGV SRHCTSSSWS RAQLHGASEE PGHFSLTNAA961STHTTNEGIF SPTPGELGFS SFHRLLSGPY FWSLPSRFLG DKVTSYGGEL RFTVTQRSQP1021GSTPLHGQPL VVLQGNNIIL EHHVAQEPSP GQPSTFIVPF REQAWQRPDG QPATREHLLM1081ALAGIDTLLI RASYAQQPAE SRVSGISMDV AVPEETGQDP ALEVEQCSCP PGYRGPSCQD1141CDTGYTRTPS GLYLGTCERC SCHGHSEACE PETGACQGCQ HHTEGPRCEQ CQPGYYGDAQ1201RGTPQDCQLC PCYGDPAAGQ AAHTCFLDTD GHPTCDACSP GHSGRHCERC APGYYGNPSQ1261GQPCQRDSQV PGPIGCNCDP QGSVSSQCDA AGQCQCKAQV EGLTCSHCRP HHFHLSASNP1321DGCLPCFCMG ITQQCASSAY TRHLISTHFA PGDFQGFALV NPQRNSRLTG EFTVEPVPEG1381AQLSFGNFAQ LGHESFYWQL PETYQGDKVA AYGGKLRYTL SYTAGPQGSP LSDPDVQITG1441NNIMLVASQP ALQGPERRSY EIMFREEFWR RPDGQPATRE HLLMALADLD ELLIRATFSS1501VPLVASISAV SLEVAQPGPS NRPRALEVEE CRCPPGYIGL SCQDCAPGYT RTGSGLYLGH1561CELCECNGHS DLCHPETGAC SQCQHNAAGE FCELCAPGYY GDATAGTPED CQPCACPLTN1621PENMFSRTCE SLGAGGYRCT ACEPGYTGQY CEQCGPGYVG NPSVQGGQCL PETNQAPLVV1681EVHPARSIVP QGGSHSLRCQ VSGSPPHYFY WSREDGRPVP SGTQQRHQGS ELHFPSVQPS1741DAGVYICTCR NLHQSNTSRA ELLVTEAPSK PITVTVEEQR SQSVRPGADV TFICTAKSKS1801PAYTLVWTRL HNGKLPTRAM DFNGILTIRN VQLSDAGTYV CTGSNMFAMD QGTATLHVQA1861SGTLSAPVVS IHPPQLTVQP GQLAEFRCSA TGSPTPTLEW TGGPGGQLPA KAQIHGGILR1921LPAVEPTDQA QYLCRAHSSA GQQVARAVLH VHGGGGPRVQ VSPERTQVHA GRTVRLYCRA1981AGVPSATITW RKEGGSLPPQ ARSERTDIAT LLIPAITTAD AGFYLCVATS PAGTAQARIQ2041VVVLSASDAS PPPVKIESSS PSVTEGQTLD LNCVVAGSAH AQVTWYRRGG SLPPHTQVHG2101SRLRLPQVSP ADSGEYVCRV ENGSGPKEAS ITVSVLHGTH SGPSYTPVPG STRPIRIEPS2161SSHVAEGQTL DLNCVVPGQA HAQVTWHKRG GSLPARHQTH GSLLRLHQVT PADSGEYVCH2221VVGTSGPLEA SVLVTIEASV IPGPIPPVRI ESSSSTVAEG QTLDLSCVVA GQAHAQVTWY2281KRGGSLPARH QVRGSRLYIF QASPADAGQY VCRASNGMEA SITVTVTGTQ GANLAYPAGS2341TQPIRIEPSS SQVAEGQTLD LNCVVPGQSH AQVTWHKRGG SLPVRHQTHG SLLRLYQASP2401ADSGEYVCRV LGSSVPLEAS VLVTIEPAGS VPALGVTPTV RIESSSSQVA EGQTLDLNCL2461VAGQAHAQVT WHKRGGSLPA RHQVHGSRLR LLQVTPADSG EYVCRVVGSS GTQEASVLVT2521IQQRLSGSHS QGVAYPVRIE SSSASLANGH TLDLNCLVAS QAPHTITWYK RGGSLPSRHQ2581IVGSRLRIPQ VTPADSGEYV CHVSNGAGSR ETSLIVTIQG SGSSHVPSVS PPIRIESSSP2641TVVEGQTLDL NCVVARQPQA IITWYKRGGS LPSRHQTHGS HLRLHQMSVA DSGEYVCRAN2701NNIDALEASI VISVSPSAGS PSAPGSSMPI RIESSSSHVA EGETLDLNCV VPGQAHAQVT2761WHKRGGSLPS HHQTRGSRLR LHHVSPADSG EYVCRVMGSS GPLEASVLVT IEASGSSAVH2821VPAPGGAPPI RIEPSSSRVA EGQTLDLKCV VPGQAHAQVT WHKRGGNLPA RHQVHGPLLR2881LNQVSPADSG EYSCQVTGSS GTLEASVLVT IEPSSPGPIS APGLAQPIYI EASSSHVTEG2941QTLDLNCVVP GQAHAQVTWY KRGGSLPARH QTHGSQLRLH LVSPADSGEY VCRAASGPGP3001EQEASFTVTV PPSEGSSYRL RSPVISIDPP SSTVQQGQDA SFKCLIHDGA APISLEWKTR3061NQELEDNVHI SPNGSIITIV GTRPSNHGTY RCVASNAYGV AQSVVNLSVH GPPTVSVLPE3121GPVWVKVGKA VTLECVSAGE PRSSARWTRI SSTPAKLEQR TYGLMDSHAV LQISSAKPSD3181AGTYVCLAQN ALGTAQKQVE VIVDTGAMAP GAPQVQAEEA ELTVEAGHTA TLRCSATGSP3241APTIHWSKLR SPLPWQYRLE GDTLIIPRVA QQDSGQYICN ATSPAGHAEA TIILHVESPP3301YATTVPEHAS VQAGETVQLQ CLAHGTPPLT FQWSRVGSSL PGRATARNEL LHFERAAPED3361SGRYRCRVTN KVGSAEAFAQ LLVQGPPGSL PATSIPAGST PTVQVTPQLE TKSIGASVEF3421HCAVPSDRGT QLRWFKEGGQ LPPGHSVQDG VLRIQNLDQS CQGTYICQAH GPWGKAQASA3481QLVIQALPSV LINIRTSVQT VVVGHAVEFE CLALGDPKPQ VTWSKVGGHL RPGIVQSGGV3541VRIAHVELAD AGQYRCTATN AAGTTQSHVL LLVQALPQIS MPQEVRVPAG SAAVFPCIAS3601GYPTPDISWS KLDGSLPPDS RLENNMLMLP SVRPQDAGTY VCTATNRQGK VKAFAHLQVP3661ERVVPYFTQT PYSFLPLPTI KDAYRKFEIK ITFRPDSADG MLLYNGQKRV PGSPTNLANR3721QPDFISFGLV GGRPEFRFDA GSGMATIRHP TPLALGHFHT VTLLRSLTQG SLIVGDLAPV3781NGTSQGKFQG LDLNEELYLG GYPDYGAIPK AGLSSGFIGC VRELRIQGEE IVFHDLNLTA3841HGISHCPTCR DRPCQNGGQC HDSESSSYVC VCPAGFTGSR CEHSQALHCH PEACGPDATC3901VNRPDGRGYT CRCHLGRSGL RCEEGVTVTT PSLSGAGSYL ALPALTNTHH ELRLDVEFKP3961LAPDGVLLFS GGKSGPVEDF VSLAMVGGHL EFRYELGSGL AVLRSAEPLA LGRWHRVSAE4021RLNKDGSLRV NGGRPVLRSS PGKSQGLNLH TLLYLGGVEP SVPLSPATNM SAHFRGCVGE4081VSVNGKRLDL TYSFLGSQGI GQCYDSSPCE RQPCQHGATC MPAGEYEFQC LCRDGFKGDL4141CEHEENPCQL REPCLHGGTC QGTRCLCLPG FSGPRCQQGS GHGIAESDWH LEGSGGNDAP4201GQYGAYFHDD GFLAFPGHVF SRSLPEVPET IELEVRTSTA SGLLLWQGVE VGEAGQGKDF4261ISLGLQDGHL VFRYQLGSGE ARLVSEDPIN DGEWHRVTAL REGRRGSIQV DGEELVSGRS4321PGPNVAVNAK GNVYIGGAPD VATLTGGRFS SGITGCVKNL VLHSARPGAP PPQPLDLQHR4381AQAGANTRPC PSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
4392 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.