Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HSPC304 / C7orf64 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109010-100
Catalog Number: LS-G109010
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RBM48 (NM_032120), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C7orf64 | DKFZP564O0523 | HSPC304 | RNA binding motif protein 48 | UPF0712 protein C7orf64 | RGD1310794 | RNA-binding protein 48 | RBM48
- Origin Species
- Human
- NCBI GenBank
- NM_032120
- SwissProt
- Q5RL73
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASSGGELGS LFDHHVQRAV CDTRAKYREG RRPRAVKVYT INLESQYLLI QGVPAVGVMK61ELVERFALYG AIEQYNALDE YPAEDFTEVY LIKFMNLQSA RTAKRKMDEQ SFFGGLLHVC121YAPEFETVEE TRKKLQMRKA YVVKTTENKD HYVTKKKLVT EHKDTEDFRQ DFHSEMSGFC181KAALNTSAGN SNPYLPYSCE LPLCYFSSKC MCSSGGPVDR APDSSKDGRN HHKTMGHYNH241NDSLRKTQIN SLKNSVACPG AQKAITSSEA VDRFMPRTTQ LQERKRRRED DRKLGTFLQT301NPTGNEIMIG PLLPDISKVD MHDDSLNTTA NLIRHKLKEV ISSVPKPPED KPEDVHTSHP361LKQRRRITRTRPLEQKLISEEDLAANDILDYKDDDDKV
367 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.