Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HSPC167 / CDK5RAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112677-100
Catalog Number: LS-G112677
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CDK5RAP1 (NM_016408), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDK5RAP1 | CDK5RAP1.3 | C20orf34 | C42 | CDK5RAP1.4 | CGI-05 | HSPC167
- Origin Species
- Human
- NCBI GenBank
- NM_016082
- SwissProt
- Q96SZ6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MHPLQCVLQV QRSLGWGPLA SVSWLSLRMC RAHSSLSSTM CPSPERQEDG ARKDFSSRLA61AGPTFQHFLK SASAPQEKLS SEVEDPPPYL MMDELLGRQR KVYLETYGCQ MNVNDTEIAW121SILQKSGYLR TSNLQEADVI LLVTCSIREK AEQTIWNRLH QLKALKTRRP RSRVPLRIGI181LGCMAERLKE EILNREKMVD ILAGPDAYRD LPRLLAVAES GQQAANVLLS LDETYADVMP241VQTSASATSA FVSIMRGCDN MCSYCIVPFT RGRERSRPIA SILEEVKKLS EQGLKEVTLL301GQNVNSFRDN SEVQFNSAVP TNLSRGFTTN YKTKQGGLRF AHLLDQVSRV DPEMRIRFTS361PHPKDFPDEV LQLIHERDNI CKQIHLPAQS GSSRVLEAMR RGYSREAYVE LVHHIRESIP421GVSLSSDFIA GFCGETEEDH VQTVSLLREV QYNMGFLFAY SMRQKTRAYH RLKDDVPEEV481KLRRLEELIT IFREEATKAN QTSVGCTQLV LVEGLSKRSA TDLCGRNDGN LKVIFPDAEM541EDVNNPGLRV RAQPGDYVLV KITSASSQTL RGHVLCRTTL RDSSAYCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
587 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.