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HSPA13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HSPA13 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HSPA13 antibodies →
$504.00each
Catalog Number: LS-G100950-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human STCH(HSPA13) (NM_006948), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
STCH | Heat shock 70 kDa protein 13 | HSPA13
Origin Species
Human
NCBI GenBank
NM_006948
SwissProt
P48723

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.99 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAREMTILGS AVLTLLLAGY LAQQYLPLPT PKVIGIDLGT TYCSVGVFFP GTGKVKVIPD
61ENGHISIPSM VSFTDNDVYV GYESVELADS NPQNTIYDAK RFIGKIFTAE ELEAEIGRYP
121FKVLNKNGMV EFSVTSNETI TVSPEYVGSR LLLKLKEMAE AYLGMPVANA VISVPAEFDL
181KQRNSTIEAA NLAGLKILRV INEPTAAAMA YGLHKADVFH VLVIDLGGGT LDVSLLNKQG
241GMFLTRAMSG NNKLGGQDFN QRLLQYLYKQ IYQTYGFVPS RKEEIHRLRQ AVEMVKLNLT
301LHQSAQLSVL LTVEEQDRKE PHSSDTELPK DKLSSADDHR VNSGFGRGLS DKKSGESQVL
361FETEISRKLF DTLNEDLFQK ILVPIQQVLK EGHLEKTEID EVVLVGGSTR IPRIRQVIQE
421FFGKDPNTSV DPDLAVVTGV AIQAGIDGGF WPLQVSALEI PNKHLQKTNF N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

471 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.