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HSPA12B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HSPA12B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HSPA12B antibodies →
$504.00each
Catalog Number: LS-G108023-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HSPA12B (NM_052970), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_052970

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLAVPEMGLQ GLYIGSSPER SPVPSPPGSP RTQESCGIAP LTPSQSPKPE VRAPQQASFS
61VVVAIDFGTT SSGYAFSFAS DPEAIHMMRK WEGGDPGVAH QKTPTCLLLT PEGAFHSFGY
121TARDYYHDLD PEEARDWLYF EKFKMKIHSA TDLTLKTQLE AVNGKTMPAL EVFAHALRFF
181REHALQELRE QSPSLPEKDT VRWVLTVPAI WKQPAKQFMR EAAYLAGLVS RENAEQLLIA
241LEPEAASVYC RKLRLHQLLD LSGRAPGGGR LGERRSIDSS FRQAREQLRR SRHSRTFLVE
301SGVGELWAEM QAGDRYVVAD CGGGTVDLTV HQLEQPHGTL KELYKASGGP YGAVGVDLAF
361EQLLCRIFGE DFIATFKRQR PAAWVDLTIA FEARKRTAGP HRAGALNISL PFSFIDFYRK
421QRGHNVETAL RRSSVNFVKW SSQGMLRMSC EAMNELFQPT VSGIIQHIEA LLARPEVQGV
481KLLFLVGGFA ESAVLQHAVQ AALGARGLRV VVPHDVGLTI LKGAVLFGQA PGVVRVRRSP
541LTYGVGVLNR FVPGRHPPEK LLVRDGRRWC TDVFERFVAA EQSVALGEEV RRSYCPARPG
601QRRVLINLYC CAAEDARFIT DPGVRKCGAL SLELEPADCG QDTAGAPPGR REIRAAMQFG
661DTEIKVTAVD VSTNRSVRAS IDFLSN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

686 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.