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HSPA12A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HSPA12A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HSPA12A antibodies →
$725.00each
Catalog Number: LS-G109609-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HSPA12A (NM_025015), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Heat shock 70 kDa protein 12A | Heat shock 70kD protein 12A | HSPA12A | Heat shock 70kDa protein 12A | KIAA0417
Origin Species
Human
NCBI GenBank
AB007877
SwissProt
O43301

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MADKEAGGSD GPRETAPTSA YSSPARSLGD TGITPLSPSH IVNDTDSNVS EQQSFLVVVA
61VDFGTTSSGY AYSFTKEPEC IHVMRRWEGG DPGVSNQKTP TTILLTPERK FHSFGYAARD
121FYHDLDPNEA KQWLYLEKFK MKLHTTGDLT MDTDLTAANG KKVKALEIFA YALQYFKEQA
181LKELSDQAGS EFENSDVRWV ITVPAIWKQP AKQFMRQAAY QAGLASPENS EQLIIALEPE
241AASIYCRKLR LHQMIELSSK AAVNGYSGSD TVGAGFTQAK EHIRRNRQSR TFLVENVIGE
301IWSELEEGDK YVVVDSGGGT VDLTVHQIRL PEGHLKELYK ATGGPYGSLG VDYEFEKLLY
361KIFGEDFIEQ FKIKRPAAWV DLMIAFESRK RAAAPDRTNP LNITLPFSFI DYYKKFRGHS
421VEHALRKSNV DFVKWSSQGM LRMSPDAMNA LFKPTIDSII EHLRDLFQKP EVSTVKFLFL
481VGGFAEAPLL QQAVQAAFGD QCRIIIPQDV GLTILKGAVL FGLDPAVIKV RRSPLTYGVG
541VLNRYVEGKH PPEKLLVKDG TRWCTDVFDK FISADQSVAL GELVKRSYTP AKPSQLVIVI
601NIYSSEHDNV SFITDPGVKK CGTLRLDLTG TSGTAVPARR EIQTLMQFGD TEIKATAIDI
661ATSKSVKVGI DFLNY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

675 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.