Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HSP90AA1 / Hsp90 Alpha A1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G99284-100
Catalog Number: LS-G99284
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HSP90AA1 (NM_001017963), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- EL52 | HSP86 | Hsp89 | HSP89A | HSPC1 | Heat shock 86 kDa | Hsp90 | HSP90A | HSP90AA1 | HSP90N | HSPCA | HSPCAL4 | HSPN | LAP2 | HSP 86 | HSPCAL1
- NCBI GenBank
- NM_005348
- SwissProt
- P07900
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPPCSGGDGS TPPGPSLRDR DCPAQSAEYP RDRLDPRPGS PSEASSPPFL RRAPVNWYQE61KAQVFLWHLL VSGSTTLLCL WKQPFHVSAF PVTASLAFRQ SQGAGQHLYK DLQPFILLRL121LMPEETQTQD QPMEEEEVET FAFQAEIAQL MSLIINTFYS NKEIFLRELI SNSSDALDKI181RYESLTDPSK LDSGKELHIN LIPNKQDRTL TIVDTGIGMT KADLINNLGT IAKSGTKAFM241EALQAGADIS MIGQFGVGFY SAYLVAEKVT VITKHNDDEQ YAWESSAGGS FTVRTDTGEP301MGRGTKVILH LKEDQTEYLE ERRIKEIVKK HSQFIGYPIT LFVEKERDKE VSDDEAEEKE361DKEEEKEKEE KESEDKPEIE DVGSDEEEEK KDGDKKKKKK IKEKYIDQEE LNKTKPIWTR421NPDDITNEEY GEFYKSLTND WEDHLAVKHF SVEGQLEFRA LLFVPRRAPF DLFENRKKKN481NIKLYVRRVF IMDNCEELIP EYLNFIRGVV DSEDLPLNIS REMLQQSKIL KVIRKNLVKK541CLELFTELAE DKENYKKFYE QFSKNIKLGI HEDSQNRKKL SELLRYYTSA SGDEMVSLKD601YCTRMKENQK HIYYITGETK DQVANSAFVE RLRKHGLEVI YMIEPIDEYC VQQLKEFEGK661TLVSVTKEGL ELPEDEEEKK KQEEKKTKFE NLCKIMKDIL EKKVEKVVVS NRLVTSPCCI721VTSTYGWTAN MERIMKAQAL RDNSTMGYMA AKKHLEINPD HSIIETLRQK AEADKNDKSV781KDLVILLYET ALLSSGFSLE DPQTHANRIY RMIKLGLGID EDDPTADDTS AAVTEEMPPL841EGDDDTSRME EVDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
853 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.