Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HSD17B10 / HADH2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100311-100
Catalog Number: LS-G100311
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ERAB(HSD17B10) (NM_004493), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 17-beta-HSD 10 | 17b-HSD10 | CAMR | DUPXp11.22 | ABAD | ERAB | HADH2 | MHBD | MRPP2 | MRX17 | SCHAD | SDR5C1 | MRX31 | MRXS10 | Type II HADH | HCD2 | HSD17B10 | XH98G2
- Origin Species
- Human
- NCBI GenBank
- NM_004493
- SwissProt
- Q99714
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 26.92 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAACRSVKG LVAVITGGAS GLGLATAERL VGQGASAVLL DLPNSGGEAQ AKKLGNNCVF61APADVTSEKD VQTALALAKG KFGRVDVAVN CAGIAVASKT YNLKKGQTHT LEDFQRVLDV121NLMGTFNVIR LVAGEMGQNE PDQGGQRGVI INTASVAAFE GQVGQAAYSA SKGGIVGMTL181PIARDLAPIG IRVMTIAPGL FGTPLLTSLP EKVCNFLASQ VPFPSRLGDP AEYAHLVQAI241IENPFLNGEV IRLDGAIRMQ PTRTRPLEQK LISEEDLAAN DILDYKDDDD KV
292 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.