Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HSD-1 / SPAG8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105437-100
Catalog Number: LS-G105437
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPAG8 (NM_172312), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Sperm membrane protein BS-84 | Sperm-associated antigen 8 | SMP-1 | Sperm associated antigen 8 | SMP1 | SPAG3 | BS-84 | HSD-1 | HSMP-1 | SPAG8 | Sperm membrane protein 1
- Origin Species
- Human
- NCBI GenBank
- BC019247
- SwissProt
- Q99932
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1METNGSTEGS RSRSRSLDIQ PSSEGLGPTS EPFPSSDDSP RSALAAATAA AAAAASAAAA61TAAFTTAKAA ALSTKTPAPC SEFMEPSSDP SLLGEPCAGP GFTHNIAHGS LGFEPVYVSC121IAQDTCTTTD HSSNPGPVPG SSSGPVLGSS SGAGHGSGSG SGPGCGSVPG SGSGPGPGSG181PGSGPGHGSG SHPGPASGPG PDTGPDSELS PCIPPGFRNL VADRVLNYTS WSQHCPWEPQ241KQPPWEFLQV LEPGARGLWK PPDIKGKLMV CYETLPRGQC LLYNWEEERA TNHLDQVPSM301QDGSESFFFR HGHRGLLTMQ LKSPMPSSTT QKDSYQPPGN VYWPLRGKRE AMLEMLLQHQ361ICKEVQAEQE PTRKLFEVES VTHHDYRMEL AQAGTPAPTK PHDYRQEQPE TFWIQRAPQL421PTWWPLPTQV PAAEDYLTWK EWGFTGVQEV LSALLRATPG EYSVNICGMN EHPVCSRTWT481NRLCHQEMGS KKTVTQEDRG WTRTRPLEQKLISEEDLAANDILDYKDDDDKV
501 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.