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HS3ST3B1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HS3ST3B1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G115577-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HS3ST3B1 (NM_006041), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
30ST3B1 | 3OST3B1 | 3-OST-3B | H3-OST-3B | HS3ST3B | HS3ST3B1
Origin Species
Human
NCBI GenBank
NM_006041

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
43.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGQRLSGGRS CLDVPGRLLP QPPPPPPPVR RKLALLFAML CVWLYMFLYS CAGSCAAAPG
61LLLLGSGSRA AHDPPALATA PDGTPPRLPF RAPPATPLAS GKEMAEGAAS PEEQSPEVPD
121SPSPISSFFS GSGSKQLPQA IIIGVKKGGT RALLEFLRVH PDVRAVGAEP HFFDRSYDKG
181LAWYRDLMPR TLDGQITMEK TPSYFVTREA PARISAMSKD TKLIVVVRDP VTRAISDYTQ
241TLSKRPDIPT FESLTFKNRT AGLIDTSWSA IQIGIYAKHL EHWLRHFPIR QMLFVSGERL
301ISDPAGELGR VQDFLGLKRI ITDKHFYFNK TKGFPCLKKA EGSSRPHCLG KTKGRTHPEI
361DREVVRRLRE FYRPFNLKFY QMTGHDFGWD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

390 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.