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HS1BP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HS1BP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110362-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HS1BP3 (NM_022460), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HCLS1-binding protein 3 | HCLS1 binding protein 3 | HS1-BP3 | HS1BP3 | HSP1BP-3 | HS1-binding protein 3 | ETM2
Origin Species
Human
NCBI GenBank
NM_022460
SwissProt
Q53T59

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
42.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQSPAVLVTS RRLQNAHTGL DLTVPQHQEV RGKMMSGHVE YQILVVTRLA AFKSAKHRPE
61DVVQFLVSKK YSEIEEFYQK LSSRYAAASL PPLPRKVLFV GESDIRERRA VFNEILRCVS
121KDAELAGSPE LLEFLGTRSP GAAGLTSRDS SVLDGTDSQT GNDEEAFDFF EEQDQVAEEG
181PPVQSLKGED AEESLEEEEA LDPLGIMRSK KPKKHPKVAV KAKPSPRLTI FDEEVDPDEG
241LFGPGRKLSP QDPSEDVSSM DPLKLFDDPD LGGAIPLGDS LLLPAACESG GPTPSLSHRD
301ASKELFRVEE DLDQILNLGA EPKPKPQLKP KPPVAAKPVI PRKPAVPPKA GPAEAVAGQQ
361KPQEQIQAMD EMDILQYIQD HDTPAQATPS LF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

392 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.