Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HRH4 / Histamine H4 Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110661-100
Catalog Number: LS-G110661
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HRH4 (NM_021624), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BG26 | G-protein coupled receptor 105 | GPRv53 | H4 | HH4R | Histamine H4 receptor | HRH4 | GPCR105 | H4R | Pfi-013 | SP9144 | AXOR35 | Gprc105 | H4 histamine receptor | Histamine receptor H4
- Origin Species
- Human
- NCBI GenBank
- AY136745
- SwissProt
- Q9H3N8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPDTNSTINL SLSTRVTLAF FMSLVAFAIM LGNALVILAF VVDKNLRHRS SYFFLNLAIS61DFFVGVISIP LYIPHTLFEW DFGKEICVFW LTTDYLLCTA SVYNIVLISY DRYLSVSNAV121SYRTQHTGVL KIVTLMVAVW VLAFLVNGPM ILVSESWKDE GSECEPGFFS EWYILAITSF181LEFVIPVILV AYFNMNIYWS LWKRDHLSRC QSHPGLTAVS SNICGHSFRG RLSSRRSLSA241STEVPASFHS ERQRRKSSLM FSSRTKMNSN TIASKMGSFS QSDSVALHQR EHVELLRARR301LAKSLAILLG VFAVCWAPYS LFTIVLSFYS SATGPKSVWY RIAFWLQWFN SFVNPLLYPL361CHKRFQKAFL KIFCIKKQPL PSQHSRSVSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
390 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.