Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HRAS / H-Ras Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104865-100
Catalog Number: LS-G104865
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human p21 Ras(HRAS) (NM_176795), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C-H-RAS | C-has/bas p21 protein | C-ras-Ki-2 activated oncogene | C-HA-RAS1 | CTLO | H-ras | H-RASIDX | GTP- and GDP-binding peptide B | GTPase HRas | H-Ras-1 | HAMSV | HRAS | HRAS1 | K-RAS | Ha-Ras | Ha-Ras1 proto-oncoprotein | Transforming protein p21 | N-ras | p21ras | RASH1 | C-BAS/HAS | C-ha-ras | p19 H-RasIDX protein
- Origin Species
- Human
- NCBI GenBank
- NM_005343
- SwissProt
- P01112
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 18.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG61QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL121AARTVESRQA QDLARSYGIP YIETSAKTRQ GSRSGSSSSS GTLWDPPGPMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
170 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.