Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HPT / MRS2L Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111082-100
Catalog Number: LS-G111082
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MRS2 (NM_020662), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MRS2 | MRS2-like protein | HPT | MRS2L
- Origin Species
- Human
- NCBI GenBank
- NM_020662
- SwissProt
- Q9HD23
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MECLRSLPCL LPRAMRLPRR TLCALALDVT SVGPPVAACG RRANLIGRSR AAQLCGPDRL61RVAGEVHRFR TSDVSQATLA SVAPVFTVTK FDKQGNVTSF ERKKTELYQE LGLQARDLRF121QHVMSITVRN NRIIMRMEYL KAVITPECLL ILDYRNLNLE QWLFRELPSQ LSGEGQLVTY181PLPFEFRAIE ALLQYWINTL QGKLSILQPL ILETLDALVD PKHSSVDRSK LHILLQNGKS241LSELETDIKI FKESILEILD EEELLEELCV SKWSDPQVFE KSSAGIDHAE EMELLLENYY301RLADDLSNAA RELRVLIDDS QSIIFINLDS HRNVMMRLNL QLTMGTFSLS LFGLMGVAFG361MNLESSLEED HRIFWLITGI MFMGSGLIWR RLLSFLGRQL EAPLPPMMAS LPKKTLLADR421SMELKNSLRL DGLGSGRSIL TNRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
443 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.