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HPS5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HPS5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HPS5 antibodies →
$725.00each
Catalog Number: LS-G104432-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HPS5 (NM_181507), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AIBP63 | HPS5 | KIAA1017 | Ru2 | Hermansky-Pudlak syndrome 5 | Ruby-eye protein 2 homolog
Origin Species
Human
NCBI GenBank
NM_007216
SwissProt
Q9UPZ3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
127.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAFVPVIPES YSHVLAEFES LDPLLSALRL DSSRLKCTSI AVSRKWLALG SSGGGLHLIQ
61KEGWKHRLFL SHREGAISQV ACCLHDDDYV AVATSQGLVV VWELNQERRG KPEQMYVSSE
121HKGRRVTALC WDTAILRVFV GDHAGKVSAI KLNTSKQAKA AAAFVMFPVQ TITTVDSCVV
181QLDYLDGRLL ISSLTRSFLC DTEREKFWKI GNKERDGEYG ACFFPGRCSG GQQPLIYCAR
241PGSRMWEVNF DGEVISTHQF KKLLSLPPLP VITLRSEPQY DHTAGSSQSL SFPKLLHLSE
301HCVLTWTERG IYIFIPQNVQ VLLWSEVKDI QDVAVCRNEL FCLHLNGKVS HLSLISVERC
361VERLLRRGLW NLAARTCCLF QNSVIASRAR KTLTADKLEH LKSQLDHGTY NDLISQLEEL
421ILKFEPLDSA CSSRRSSISS HESFSILDSG IYRIISSRRG SQSDEDSCSL HSQTLSEDER
481FKEFTSQQEE DLPDQCCGSH GNEDNVSHAP VMFETDKNET FLPFGIPLPF RSPSPLVSLQ
541AVKESVSSFV RKTTEKIGTL HTSPDLKVRP ELRGDEQSCE EDVSSDTCPK EEDTEEEKEV
601TSPPPEEDRF QELKVATAEA MTKLQDPLVL FESESLRMVL QEWLSHLEKT FAMKDFSGVS
661DTDNSSMKLN QDVLLVNESK KGILDEDNEK EKRDSLGNEE SVDKTACECV RSPRESLDDL
721FQICSPCAIA SGLRNDLAEL TTLCLELNVL NSKIKSTSGH VDHTLQQYSP EILACQFLKK
781YFFLLNLKRA KESIKLSYSN SPSVWDTFIE GLKEMASSNP VYMEMEKGDL PTRLKLLDDE
841VPFDSPLLVV YATRLYEKFG ESALRSLIKF FPSILPSDII QLCHHHPAEF LAYLDSLVKS
901RPEDQRSSFL ESLLQPESLR LDWLLLAVSL DAPPSTSTMD DEGYPRPHSH LLSWGYSQLI
961LHLIKLPADF ITKEKMTDIC RSCGFWPGYL ILCLELERRR EAFTNIVYLN DMSLMEGDNG
1021WIPETVEEWK LLLHLIQSKS TRPAPQESLN GSLSDGPSPI NVENVALLLA KAMGPDRAWS
1081LLQECGLALE LSEKFTRTCD ILRIAEKRQR ALIQSMLEKC DRFLWSQQA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1129 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.