Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HPN / TMPRSS1 / Hepsin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104062-100
Catalog Number: LS-G104062
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Hepsin(HPN) (NM_182983), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hepsin | HPN | TMPRSS1 | Serine protease hepsin
- Origin Species
- Human
- NCBI GenBank
- NM_002151
- SwissProt
- P05981
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQKEGGRTV PCCSRPKVAA LTAGTLLLLT AIGAASWAIV AVLLRSDQEP LYPVQVSSAD61ARLMVFDKTE GTWRLLCSSR SNARVAGLSC EEMGFLRALT HSELDVRTAG ANGTSGFFCV121DEGRLPHTQR LLEVISVCDC PRGRFLAAIC QDCGRRKLPV DRIVGGRDTS LGRWPWQVSL181RYDGAHLCGG SLLSGDWVLT AAHCFPERNR VLSRWRVFAG AVAQASPHGL QLGVQAVVYH241GGYLPFRDPN SEENSNDIAL VHLSSPLPLT EYIQPVCLPA AGQALVDGKI CTVTGWGNTQ301YYGQQAGVLQ EARVPIISND VCNGADFYGN QIKPKMFCAG YPEGGIDACQ GDSGGPFVCE361DSISRTPRWR LCGIVSWGTG CALAQKPGVY TKVSDFREWI FQAIKTHSEA SGMVTQLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
417 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.