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HP1BP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HP1BP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112738-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HP1BP3 (NM_016287), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HP1BP3 | HP1-BP74 | Protein HP1-BP74 | RP5-930J4.3
Origin Species
Human
NCBI GenBank
NM_016287
SwissProt
Q5SSJ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATDTSQGEL VHPKALPLIV GAQLIHADKL GEKVEDSTMP IRRTVNSTRE TPPKSKLAEG
61EEEKPEPDIS SEESVSTVEE QENETPPATS SEAEQPKGEP ENEEKEENKS SEETKKDEKD
121QSKEKEKKVK KTIPSWATLS ASQLARAQKQ TPMASSPRPK MDAILTEAIK ACFQKSGASV
181VAIRKYIIHK YPSLELERRG YLLKQALKRE LNRGVIKQVK GKGASGSFVV VQKSRKTPQK
241SRNRKNRSSA VDPEPQVKLE DVLPLAFTRL CEPKEASYSL IRKYVSQYYP KLRVDIRPQL
301LKNALQRAVE RGQLEQITGK GASGTFQLKK SGEKPLLGGS LMEYAILSAI AAMNEPKTCS
361TTALKKYVLE NHPGTNSNYQ MHLLKKTLQK CEKNGWMEQI SGKGFSGTFQ LCFPYYPSPG
421VLFPKKEPDD SRDEDEDEDE SSEEDSEDEE PPPKRRLQKK TPAKSPGKAA SVKQRGSKPA
481PKVSAAQRGK ARPLPKKAPP KAKTPAKKTR PSSTVIKKPS GGSSKKPATS ARKEVKLPGK
541GKSTMKKSFR VKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

553 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.