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HOOK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HOOK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101136-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HOOK2 (NM_013312), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HHK2 | Hook homolog 2 (Drosophila) | H-hook2 | HOOK2 | Protein Hook homolog 2
Origin Species
Human
NCBI GenBank
NM_013312
SwissProt
Q96ED9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSVDKAELCG SLLTWLQTFH VPSPCASPQD LSSGLAVAYV LNQIDPSWFN EAWLQGISED
61PGPNWKLKVS NLKMVLRSLV EYSQDVLAHP VSEEHLPDVS LIGEFSDPAE LGKLLQLVLG
121CAISCEKKQD HIQRIMTLEE SVQHVVMEAI QELMTKDTPD SLSPETYGNF DSQSRRYYFL
181SEEAEEGDEL QQRCLDLERQ LMLLSEEKQS LAQENAGLRE RMGRPEGEGT PGLTAKKLLL
241LQSQLEQLQE ENFRLESGRE DERLRCAELE REVAELQHRN QALTSLAQEA QALKDEMDEL
301RQSSERAGQL EATLTSCRRR LGELRELRRQ VRQLEERNAG HAERTRQLED ELRRAGSLRA
361QLEAQRRQVQ ELQGQRQEEA MKAEKWLFEC RNLEEKYESV TKEKERLLAE RDSLREANEE
421LRCAQLQPRG LTQADPSLDP TSTPVDNLAA EILPAELRET LLRLQLENKR LCRQEAADRE
481RQEELQRQLE DANRARHGLE TQHRLNQQQL SELRAQVEDL QKALQEQGGK TEDSILLKRK
541LEEHLQKLHE ADLELQRKRE YIEELEPPTD SSTARRIEEL QHNLQKKDAD LRAMEERYRR
601YVDKARMVMQ TMEPKQRPAA GAPPELHSLR TQLRERDVRI RHLEMDFEKS RSQREQEEKL
661LISAWYNMGM ALQQRAGEER APAHAQSFLA QQRLATNSRR GPLGRLASLN LRPTDKHTRT
721RPLEQKLISE EDLAANDILD YKDDDDKV

748 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.