LSBio
HOOK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HOOK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101356-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HOOK1 (NM_015888), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
H-hook1 | HHK1 | Hook homolog 1 (Drosophila) | HOOK1 | Protein Hook homolog 1
Origin Species
Human
NCBI GenBank
NM_015888
SwissProt
Q9UJC3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
84.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEETQPPPQP KLPLCDSLMI WLQTFNTASP CQDVKQLTSG VAMAQVLHQI DAAWFNESWL
61SRIKEDVGDN WRIKASNVKK VLQGIMSYYH EFLGQQISEA LIPDLNQITE CSDPVELGRL
121LQLILGCAIN CEKKQEHIQN IMTLEESVQH VVMTAIQELM SKEILSSPPN DAVGELEQQL
181KRALEELQEA LAEKEELRQR CEELDMQVTT LQDEKNSLVS ENEMMNEKLD QLDGSFDDPN
241TVVAKKYFHA QLQLEQLQEE NFRLEAAKDD YRVHCEELEK QLIEFQHRND ELTSLAEETR
301ALKDEIDVLR ATSDKANKLE STVEIYRQKL QDLNDLRKQV KTLQETNMMY MHNTVSLEEE
361LKKANAARTQ LETYKRQVQD LHVKLSSESK RADTLAFEMK RLEEKHEALL KEKERLIEQR
421DTLKETNEEL RCSQVQQDHL NQTDASATKS YENLAAEIMP VEYREVFIRL QHENKMLRLQ
481QEGSENERIE ELQEQLEQKH RKMNELETEQ RLSKERIREL QQQIEDLQKS LQEQGSKSEG
541ESSSKLKQKL EAHMEKLTEV HEELQKKQEL IEDLQPDINQ NVQKINELEA ALQKKDEDMK
601AMEERYKMYL EKARNVIKTL DPKLNPASAE IMLLRKQLAE KERRIEILES ECKVAKFRDY
661EEKLIVSAWY NKSLAFQKLG MESRLVSGGG ACSDTGACTP ARSFLAQQRH ITNTRRNLSV
721KVPATTSDTR TRPLEQKLIS EEDLAANDIL DYKDDDDKV

759 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.