LSBio
HOMEZ Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

HOMEZ Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all HOMEZ antibodies →
$725.00each
Catalog Number: LS-G110944-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HOMEZ (NM_020834), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HOMEZ | KIAA1443
Origin Species
Human
NCBI GenBank
AB037864
SwissProt
Q8IX15

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVRGWEPPPG LDCAISEGHK SEGTMPPNKE ASGLSSSPAG LICLPPISEE LQLVWTQAAQ
61TSELDSNEHL LKTFSYFPYP SLADIALLCL RYGLQMEKVK TWFMAQRLRC GISWSSEEIE
121ETRARVVYRR DQLHFKSLLS FTHHAGRPPE EVPPPPVPAP EQVGIGIGPP TLSKPTQTKG
181LKVEPEEPSQ MPPLPQSHQK LKESLMTPGS GAFPYQSDFW QHLQSSGLSK EQAGRGPNQS
241HGIGTASWNH STTVPQPQAR DKPPPIALIA SSCKEESASS VTPSSSSTSS SFQVLANGAT
301AASKPLQPLG CVPQSVSPSE QALPPHLEPA WPQGLRHNSV PGRVGPTEYL SPDMQRQRKT
361KRKTKEQLAI LKSFFLQCQW ARREDYQKLE QITGLPRPEI IQWFGDTRYA LKHGQLKWFR
421DNAVPGAPSF QDPAIPTPPP STRSLNERAE TPPLPIPPPP PDIQPLERYW AAHQQLRETD
481IPQLSQASRL STQQVLDWFD SRLPQPAEVV VCLDEEEEEE EEELPEDDEE EEEEEEEDDD
541DDDDDVIIQD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

550 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.