Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HOMER3 / Homer 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116366-100
Catalog Number: LS-G116366
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HOMER3 (NM_004838), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Homer homolog 3 (Drosophila) | HOMER3 | Homer protein homolog 3 | VESL3 | HOMER-3
- Origin Species
- Human
- NCBI GenBank
- NM_004838
- SwissProt
- Q9NSC5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSTAREQPIF STRAHVFQID PATKRNWIPA GKHALTVSYF YDATRNVYRI ISIGGAKAII61NSTVTPNMTF TKTSQKFGQW ADSRANTVYG LGFASEQHLT QFAEKFQEVK EAARLAREKS121QDGGELTSPA LGLASHQVPP SPLVSANGPG EEKLFRSQSA DAPGPTERER LKKMLSEGSV181GEVQWEAEFF ALQDSNNKLA GALREANAAA AQWRQQLEAQ RAEAERLRQR VAELEAQAAS241EVTPTGEKEG LGQGQSLEQL EALVQTKDQE IQTLKSQTGG PREALEAAER EETQQKVQDL301ETRNAELEHQ LRAMERSLEE ARAERERARA EVGRAAQLLD VRLFELSELR EGLARLAEAA361PTRTRPLEQKLISEEDLAANDILDYKDDDDKV
361 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.