Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
HNRPM / HNRNPM Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101987-100
Catalog Number: LS-G101987
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HNRPM(HNRNPM) (NM_031203), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CEA receptor | CEAR | HnRNA-binding protein M4 | HNRNPM | HNRNPM4 | HNRPM4 | N-acetylglucosamine receptor 1 | NAGR1 | HnRNP M | HNRPM | HTGR1
- Origin Species
- Human
- NCBI GenBank
- NM_005968
- SwissProt
- P52272
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAGVEAAAE VAATEIKMEE ESGAPGVPSG NGAPGPKGEG ERPAQNEKRK EKNIKRGGNR61FEPYANPTKR YRAFITNIPF DVKWQSLKDL VKEKVGEVTY VELLMDAEGK SRGCAVVEFK121MEESMKKAAE VLNKHSLSGR PLKVKEDPDG EHARRAMQKA GRLGSTVFVA NLDYKVGWKK181LKEVFSMAGV VVRADILEDK DGKSRGIGTV TFEQSIEAVQ AISMFNGQLL FDRPMHVKMD241ERALPKGDFF PPERPQQLPH GLGGIGMGLG PGGQPIDANH LNKGIGMGNI GPAGMGMEGI301GFGINKMGGM EGPFGGGMEN MGRFGSGMNM GRINEILSNA LKRGEIIAKQ GGGGGGGSVP361GIERMGPGID RLGGAGMERM GAGLGHGMDR VGSEIERMGL VMDRMGSVER MGSGIERMGP421LGLDHMASSI ERMGQTMERI GSGVERMGAG MGFGLERMAA PIDRVGQTIE RMGSGVERMG481PAIERMGLSM ERMVPAGMGA GLERMGPVMD RMATGLERMG ANNLERMGLE RMGANSLERM541GLERMGANSL ERMGPAMGPA LGAGIERMGL AMGGGGGASF DRAIEMERGN FGGSFAGSFG601GAGGHAPGVA RKACQIFVRN LPFDFTWKML KDKFNECGHV LYADIKMENG KSKGCGVVKF661ESPEVAERAC RMMNGMKLSG REIDVRIDRN ATRTRPLEQKLISEEDLAANDILDYKDDDDKV
691 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.